De novo synthesis and development of an RNA enzyme

作者: Y. Ikawa , K. Tsuda , S. Matsumura , T. Inoue

DOI: 10.1073/PNAS.0405886101

关键词: RibozymeArtificial enzymeRNA ligaseMolecular recognitionLigase ribozymeBiologyDe novo synthesisRNABiochemistryDNA ligase

摘要: Arbitrary manipulation of molecular recognition at the atomic level has many applications. However, systematic design and de novo synthesis an artificial enzyme based on such been a long-standing challenge in field chemistry biotechnology. In this report, we developed RNA ligase by implementing synthetic strategy that fuses series 3D modelings naturally occurring RNA–RNA motifs with small-scale combinatorial modular catalytic unit. The resulting produces 3′–5′ linkage template-directed manner for any combinations two nucleotides reaction site. rate is 106-fold over uncatalyzed yield higher than those previously reported ribozymes. may be applicable to development variety nonnatural functional RNAs defined structures.

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