作者: Y. Ikawa , K. Tsuda , S. Matsumura , T. Inoue
关键词: Ribozyme 、 Artificial enzyme 、 RNA ligase 、 Molecular recognition 、 Ligase ribozyme 、 Biology 、 De novo synthesis 、 RNA 、 Biochemistry 、 DNA ligase
摘要: Arbitrary manipulation of molecular recognition at the atomic level has many applications. However, systematic design and de novo synthesis an artificial enzyme based on such been a long-standing challenge in field chemistry biotechnology. In this report, we developed RNA ligase by implementing synthetic strategy that fuses series 3D modelings naturally occurring RNA–RNA motifs with small-scale combinatorial modular catalytic unit. The resulting produces 3′–5′ linkage template-directed manner for any combinations two nucleotides reaction site. rate is 106-fold over uncatalyzed yield higher than those previously reported ribozymes. may be applicable to development variety nonnatural functional RNAs defined structures.