Wnt/β-catenin up-regulates Midkine expression in glioma cells.
作者： Shi-Lei Tang , Xiao-Bing Chen , Yuan-Lin Gao
DOI:
关键词: Transfection 、 Wnt signaling pathway 、 Catenin 、 Chromatin immunoprecipitation 、 Bioinformatics 、 Neurite 、 WNT3A 、 Midkine 、 Cancer research 、 Glioma 、 Biology
摘要: Midkine, also known as neurite growth-promoting factor 2 (NEGF2), plays an important role in cell proliferation, apoptosis and differentiation. Recent studies have shown that Midkine is up-regulated several types of human cancers. However, the molecular mechanism for its up-regulation remains poorly understood. Activation Wnt/β-catenin signaling viewed crucial multiple tumor growth metastasis, including glioma. In present study, we found Wnt3a administration or transfection a constitutively activated β-catenin promoted expression glioma cells. We further identified TCF/LEF binding site, with which beta-catenin interacts, on proximal promoter region gene, by luciferase reporter chromatin immunoprecipitation assays. Thus, our results suggest previously unknown Wnt/β-catenin/Midkine network controlling development.
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