Plant virus serology.

作者: M.H.V. van Regenmortel

DOI: 10.1016/S0065-3527(08)60850-7

关键词: PrecipitinBiologyVirusVirologyVirus classificationPlant virusAntiserumAntigenPlant VirologyImmunodiffusion

摘要: Publisher Summary The accelerated development of serological studies on plant viruses during the past ten years can be followed easily by simply counting number that have proved amenable to testing. This steady increase partly reflects widening applicability and improvements virus purification methods seems allow generalization eventually antisera will become available all mechanically transmissible viruses, whose nucleic acid is protected a protein coat. increasing popularity also closely upon immunodiffusion techniques greatly facilitated work. Most early immunological were performed laborious precipitin reaction in free liquid. Improvements methods, production high-tittered their use classification, acceptance criteria nomenclature, as models for study some unsolved riddles immunochemistry. most commonly used test virology without question reaction. Gel tests are less than those liquid, they require antiserum, provide more information homogeneity purity reactants size relationships among antigens. Two main groups gel diffusion usually distinguished: simple (or single) where only one reactants, antigen, diffusing into containing other reactant, double both initially them. Each group further divided dimension two dimensions, depending whether reactions carried out tubes or plates.

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