Nanos1 functions as a translational repressor in the Xenopus germline.

摘要: Nanos family members have been shown to act as translational repressors in the Drosophila and Caenorhabditis elegans germline, but direct evidence is missing for a similar function vertebrates. Using tethered assay, we show that Xenopus Nanos1 repressor association with RNA required this repression. We identified 14 amino acid region within N-terminal domain of conserved organisms diverse sponge Human. The found all vertebrates notably lacking C. elegans. Deletion substitution analysis revealed was repressive activity. Consistent observation, deletion sufficient prevent abnormal development results from ectopic expression oocytes. Although can repress capped polyadenylated RNAs, mediated repression did not require targeted cap or be polyadenylated. These suggest capable repressing translation by several different mechanisms. Nanos1, like Nanos, associates cyclin B1 vivo indicating some targets may evolutionarily conserved. protein detected thus available mRNAs while PGCs were endoderm, observed after stage.