Insights about α-tocopherol and Trolox interaction with phosphatidylcholine monolayers under peroxidation conditions through Brewster angle microscopy.

摘要: Abstract Membranes are major targets to oxidative damage, particularly due lipid oxidation, which has been associated aging. The role, efficacy and membrane interaction of antioxidants is still unclear, requiring further understanding molecular interaction. Hence, the objective this work was evaluate between (α-tocopherol its aqueous soluble analog Trolox) monolayer formed by phosphatidylcholine molecules at air/liquid interface upon peroxidation conditions, promoted peroxyl radicals from thermal decomposition 2,2′-azobis(2-methylpropionamidine) (AAPH). with three different monolayers, containing (i) 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine (DPPC), (ii) DDPC + α-linolenic acid, or (iii) egg yolk l -α-phosphatidylcholine (EPC), ascertain surface pressure (π)–molecular area (A) isotherms monitoring features through Brewster angle microscopy (BAM). DPPC monolayers confirmed modifications on domain shape for α-tocopherol maintenance typical multilobed during an extended interval Trolox. Under BAM images showed a clear components AAPH subphase changes appearance white dots, located mainly frontier condensed expanded liquid phases. White branched structures were also observed whenever both α-linolenic acid present, indicating segregation these within monolayer, highly significant in biological systems. For EPC no information obtained but π–A existence same interactions other two monolayers.