Nucleotide sequence of the gene coding for human factor VII, a vitamin K-dependent protein participating in blood coagulation.

摘要: Abstract Activated factor VII (factor VIIa) is a vitamin K-dependent plasma serine protease that participates in cascade of reactions leading to the coagulation blood. Two overlapping genomic clones containing sequences encoding human were isolated and characterized. The complete sequence gene was determined found span about 12.8 kilobases. mRNA for as demonstrated by cDNA cloning polyadenylylated at multiple sites but contains only one AAUAAA poly(A) signal sequence. can undergo alternative splicing, forming transcript eight segments exons another with an additional exon encodes larger prepro leader latter has no known counterpart other proteins. positions introns respect amino acid encoded essential are same those present IX, X, protein C, first three prothrombin. These code domains generally conserved among members this family. comparable these genes, however, dissimilar size sequence, exception intron C C. also five regions made up tandem repeats oligonucleotide monomer elements. More than quarter more third 3' untranslated portion consist minisatellite repeats.