Compositional and temporal stability of fecal taxon libraries for use with SourceTracker in sub-tropical catchments.
作者： Christian O'Dea , Qian Zhang , Christopher Staley , Nicole Masters , Anna Kuballa
DOI: 10.1016/J.WATRES.2019.114967
关键词: Taxon 、 Subtropics 、 Zoology 、 Amplicon sequencing 、 Feces 、 Pollution 、 16S ribosomal RNA 、 Pollutant 、 Microbial source tracking
摘要: Characterization of microbial communities using high-throughput amplicon sequencing is an emerging approach for source tracking fecal pollution. This study used SourceTracker software to examine temporal and geographical variability bacterial community profiles identify pollutant sources in three freshwater catchments sub-tropical Australia. Fecal from 10 animal species, humans, composite wastewater samples six sewage treatment plants were characterized compared Illumina the V5–V6 regions 16S rRNA gene. Source contributions calculated new taxon libraries as well previously generated determine effects geographic on assignments. determined communites within samples, shared taxonomic similarities that at low levels (typically <3%). SourceTraker also predicted occasional detection deer flying fox water samples. No significant differences observed sequences current (P ≥ 0.107). However, between newly (ANOSIM P ≤ 0.001), which <15% composition. Results suggest instability among tested highlight continual evaluation community-based MST confirmatory qPCR analyses marker genes.
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