Genome Mining and Enzymatic Total Biosynthesis of Purincyclamide.

作者： Bo Zhang , Wei Li , Yang Zhao , Rui Hua Jiao , Ren Xiang Tan

关键词: Biochemistry 、 Gene knockout 、 Biosynthesis 、 Enzyme 、 Chemistry 、 Genome mining 、 Heterologous expression 、 Gene cluster 、 Streptomyces chrestomyceticus 、 Streptomyces albus

摘要: … The CDPS (PcmA) generates two successive amide bonds by using l-Trp-tRNA and l-Tyr-tRNA as substrates, resulting in the formation of 2 as the sole product. Two enzymes encoded …