Heterogeneity and Clonal Variation Related to Cell Surface Expression of a Mouse Lung Tumor-associated Antigen Quantified Using Flow Cytometry

摘要: Previous reports have established that line 1, a spontaneous BALB/c lung carcinoma, expresses M r 180,000 tumor-associated surface antigen (TSP-180). In this study, using monoclonal antibody and flow cytometry to quantify cell TSP-180 expression, we found essentially all cells in tissue culture-adapted 1 population express TSP-180, but the amount of expressed by is quite heterogeneous. Variation was be part related size heterogeneity, expression being cycle-dependent. The surface-expressed correlated somewhat with size, greater on average for G2 cycle compartment. However, defined specific stage still showed marked heterogeneity expression. Even though per decreased during vitro propagation, little change observed. To explore whether any TSP-180-related resulted from heritable variation 263 limiting dilution-derived clones were analyzed. majority displayed, shortly after cloning, heterogeneous profiles mean levels similar those observed parent tumor. Occasionally, however, isolated again appeared as parent, differed much 3-fold Extensive passage did not substantially increase low probability isolating which TSP-180. Differences among relatively stable upon passage, typically maintained recloning, large enough influence clonal susceptibility TSP-180-directed complement-mediated lysis. Heritable some also shown independent differences cycle, or another (P-100). We concluded although demonstrating can occasionally isolated, predominantly nonheritable, present recently cloned lines, totally accounted variation.