Cryopreservation does not alter the allogenicity and development of vasculopathy in post-transplant rat aortas

摘要: Abstract Background Cryopreservation is a valuable technique for storing heart valve and vascular allografts. However, the biological ramifications of cryopreservation are still unclear; therefore, using animal experiments we assessed how ‘cryopreservation’ influences graft allogenicity cell viability. Methods Thoracic aortas Lewis rats were prepared as fresh (F) or cryopreserved (CP) grafts, implanted into infrarenal aorta Brown Norway (BNs). The grafts spleens harvested at post-operative day 7 28 (POD7, POD28) analyses. Results First, systemic immune response to transplantation was estimated by mixed lymphocyte reaction analyses spleen cells from naive recipient BNs. alloreactivity recipients increased 1.5 times that BNs POD7 POD28, when stimulated mitomycin C-treated cells. Second, local TNFα, IFNγ, iNOS mRNA expression in quantitative PCR, which revealed 20- 40-fold increases POD28 after allotransplantation. Third, endothelial viability NOS level: it similar highest F CP before then significantly decreased both syngeneic allogeneic transplantation. Finally, intimal hyperplasia, expressed I/M ratio, developed over time allotransplantation, reaching 2.5 thickness results these no difference between Conclusion did not modify allografts had minimal adverse impacts on