Potential subunit vaccine against Mycoplasma pulmonis purified by a protective monoclonal antibody.

摘要: Monoclonal antibodies (mAbs) were produced against Mycoplasma pulmonis (MP); some highly protective in the treatment of experimental infections BALB/c mice. The mAbs inhibited MP growth vitro and prevented attachment to fibroblasts or red blood cells. Three separate recognized 54-76% 54 clinical isolates MP, three together detected all isolates. We used purify antigens by affinity column chromatography. purified antigen vaccinate mice immunize rabbits capable significant inhibition sera tracheolung lavage fluids. vaccinated challenged with various doses a virulent T2 strain MP. Assays for viable organisms histopathological changes lungs infected indicated that protected if challenge dose was 10(3)-10(5), but not 10(7), c.f.u. immunized passively transfer immunity provided complete protection 1 x 10(6) conclude this protocol can provide safe vaccine disease, at least