Shikimate pathway activity in shake and fermenter cultures of Cinchona succirubra.
作者： H. -P. Schmauder , D. Gr�ger , H. Koblitz , D. Koblitz
DOI: 10.1007/BF00269365
关键词: Shikimate pathway 、 Biology 、 Context (language use) 、 Biochemistry 、 Cell culture 、 Tryptophan 、 Indole alkaloid 、 Enzyme 、 Cinchona 、 Indole test
摘要: Cell suspension cultures of Cinchona succirubra were cultivated in shake and for the first time airlift fermenters. Under both conditions L-tryptophan exerts a stimulatory effect on alkaloid formation. In this context regulatory pattern some shikimate pathway enzymes was investigated non-supplemented tryptophan supplemented cell cultures. A remarkable increase decarboxylase (TDC) activity observed cells under influence tryptophan. Apparently, like other indole producing cultures, high TDC is prerequisite Growth enzyme activities C. fermenter at controlled non-regulated pH levels followed. Optimum growth formation recorded (normal) conditions.
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