Effect of the regulation of retinoid X receptor-α gene expression on rat hepatic fibrosis
作者： Zheng Wang , Jiapeng Xu , Yongchao Zheng , Wei Chen , Yongwei Sun
DOI: 10.1111/J.1872-034X.2011.00794.X
关键词: Type I collagen 、 Endocrinology 、 Hydroxyproline 、 Retinoid 、 CCL4 、 Hepatic fibrosis 、 Biology 、 Retinoid X receptor 、 Internal medicine 、 Gene expression 、 Hepatic stellate cell 、 Hepatology 、 Infectious Diseases
摘要: Aim: To study the effect of retinoid X receptor-α (RXR-α) expression on rat hepatic fibrosis. Methods: Rat fibrosis was induced by CCl4, and rats were randomly divided into an early-phase group (2 weeks) a sustained (8 weeks). They then four groups (normal control, fibrosis, negative control RXR-α groups). A recombinant lentiviral vector carrying gene injected to induce intraportal infusion, tissue pathological examination performed, hydroxyproline content detected. Hepatic stellate cells (HSC) cultured in vitro, lentivirus used activate HSC, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) activation assayed detect HSC proliferation. Results: In vivo experiments indicated that group, there significant differences content, RXR-α, α-smooth muscle actin (α-SMA) type I collagen (P 0.05). In vitro studies revealed significantly inhibited α-SMA activated (P < 0.01), as well proliferation (P < 0.01). Conclusion: The increased degree fibrosis.
sci-hub.se 参考文章(22)
Lan Wang, Lynda Rennie Tankersley, Mei Tang, James J. Potter, Esteban Mezey, Regulation of the murine α2(I) collagen promoter by retinoic acid and retinoid X receptors Archives of Biochemistry and Biophysics. ,vol. 401, pp. 262- 270 ,(2002) , 10.1016/S0003-9861(02)00058-9
Eitan Shaulian, Michael Karin, AP-1 as a regulator of cell life and death Nature Cell Biology. ,vol. 4, ,(2002) , 10.1038/NCB0502-E131
Béatrice Durand, Michael Saunders, Pierre Leroy, Mark Leid, Pierre Chambon, All-trans and 9-cis retinoic acid induction of CRABPII transcription is mediated by RAR-RXR heterodimers bound to DR1 and DR2 repeated motifs. Cell. ,vol. 71, pp. 73- 85 ,(1992) , 10.1016/0092-8674(92)90267-G
Ross A.S. Chandraratna, Detlef Schuppan, Albert Geerts, Karine Hellemans, Peggy Verbuyst, Erik Quartier, Frans Schuit, Krista Rombouts, Differential modulation of rat hepatic stellate phenotype by natural and synthetic retinoids Hepatology. ,vol. 39, pp. 97- 108 ,(2004) , 10.1002/HEP.20015
D KNOOK, A SEFFELAAR, A DELEEUW, Fat-storing cells of the rat liver: Their isolation and purification Experimental Cell Research. ,vol. 139, pp. 468- 471 ,(1982) , 10.1016/0014-4827(82)90283-X
Hui Chen, Yong-Wen He, Wen-Qi Liu, Jing-Hui Zhang, Rosiglitazone prevents murine hepatic fibrosis induced by Schistosoma japonicum. World Journal of Gastroenterology. ,vol. 14, pp. 2905- 2911 ,(2008) , 10.3748/WJG.14.2905
Scott L. Friedman, Molecular regulation of hepatic fibrosis, an integrated cellular response to tissue injury. Journal of Biological Chemistry. ,vol. 275, pp. 2247- 2250 ,(2000) , 10.1074/JBC.275.4.2247
Michéle Chevallier, Sylviane Guerret, Philippe Chossegros, Françoise Gerard, Jean-Alexis Grimaud, A histological semiquantitative scoring system for evaluation of hepatic fibrosis in needle liver biopsy specimens: Comparison with morphometric studies Hepatology. ,vol. 20, pp. 349- 355 ,(1994) , 10.1002/HEP.1840200213
Chen Chen, Jinsheng Zhang, Jinjun Li, Jin Huang, Chen Yang, Guangcun Huang, Jianying Shi, Hydrodynamic‐based in vivo transfection of retinoic X receptor‐α gene can enhance vitamin A‐induced attenuation of liver fibrosis in mice Liver International. ,vol. 24, pp. 679- 686 ,(2004) , 10.1111/J.1478-3231.2004.0977.X
Zhennan Lai, Ina Han, Misun Park, Roscoe O. Brady, Design of an HIV-1 lentiviral-based gene-trap vector to detect developmentally regulated genes in mammalian cells Proceedings of the National Academy of Sciences of the United States of America. ,vol. 99, pp. 3651- 3656 ,(2002) , 10.1073/PNAS.062032499