In vitro and in vivo assessments of a 3-(3,4-dihydroxyphenyl)-2-propenoic acid bioconjugated gelatin-based injectable hydrogel for biomedical applications

摘要: Imparting functional properties on a biomaterial for high end applications is always challenging task. In the present study, an attempt was made to construct injectable hydrogel through bioconjugation of dihydroxy phenolic acids gelatin backbone. Bioconjugating caffeic acid with followed by oxidation mild agents provided all requisite (biocompatibility, controlled biodegradability, and antioxidant, antimicrobial wound healing ability). Bioconjugation performed using EDC/NHS resultant gel named as bioconjugated (CBG gel). The physicochemical, rheological, swelling, in vitro properties, antioxidant drug release properties) vivo biodegradability studies CBG were carried out standard protocols. confirmed 1H NMR UV-Vis analysis. Rheological analysis revealed that storage modulus greater than loss at frequencies suggested elastic nature gel. About 50% weight gain within 12 hours during swelling evaporation suitability carrier. implied there initial burst later sustained. promotes cell migration demonstrates radical scavenging behavior. When subcutaneously injected into animal, situ gel, highly biocompatible did not cause any necrosis. crosstalk adjacent tissue cells smooth completely degraded 24 days. efficacy full-thickness wounds accelerated imparted strength healed skin appreciable level. With these additional could be useful biomedical applications.