A quantitative autoradiographic study of [3H]cAMP binding to cytosolic and particulate protein kinase A in post-mortem brain staged for Alzheimer's disease neurofibrillary changes and amyloid deposits

摘要: Abstract The cAMP-dependent protein kinase (PKA) has been implicated in the Alzheimer's disease pathology of abnormal tau phosphorylation leading to neurofibrillary tangle (NFT) formation, as well amyloid precursor α-secretase processing. In present study, we determined whether [ 3 H ]cAMP binding cytosolic and particulate PKA showed any relationship extent at post-mortem. Autoradiographic was measured sections entorhinal cortex/hippocampal formation from 23 cases that had staged for disease-related changes deposits according Braak [H. Braak, E. Neuropathological staging Alzheimer's-related changes, Acta Neuropathol. 82 (1991) 239–259]. statistically significant reductions cortex (P isocortical stages (V VI), compared non-pathological (O) (by 55%, P no alterations with respect either subiculum, CA1–CA4 subfields hippocampus or dentate gyrus. also declines stage C O 41%, did not show relationships hippocampal subregions. These findings suggest whereas is reduced progression pathology, other regions a preservation even late pathologies.