The Cytochemical Localization of Oxidative Enzymes : II. Pyridine Nucleotide-Linked Dehydrogenases

摘要: Methods are presented for the intramitochondrial localization of various diphosphopyridine nucleotide and triphosphopyridine nucleotide-linked dehydrogenases in tissue sections. The cytochemical reactions studied involve oxidation substrates by a specific pyridino-protein. electron transfer tetrazolium salt is mediated diaphorase system associated with dehydrogenase. final acceptor was either p-nitrophenyl substituted ditetrazole (nitro-BT) or N-thiazol-2-yl monotetrazole (MTT), latter giving rise to metal formazan presence cobaltous ions. Mitochondrial precipitate could be achieved using hypertonic incubating media containing high concentrations substrate co-enzyme. A fast reduction obtained chemically blocking respiratory chain enzymes beyond flavoproteins. Although systems implicated salts, solely responsible distinct distribution pattern tissues substrates. present findings sections discussed conjunction existing biochemical evidence from differential centrifugation experiments.