Icariin reduces human colon carcinoma cell growth and metastasis by enhancing p53 activities.
作者： Meili Tian , Shuang Yang , Xinpeng Yan
DOI: 10.1590/1414-431X20187151
关键词: Icariin 、 Clonogenic assay 、 Small interfering RNA 、 Cytotoxicity 、 Gene knockdown 、 Apoptosis 、 Annexin 、 Cancer research 、 Chemistry 、 Blot
摘要: Icariin has been reported to possess high anticancer activity. Colon carcinoma is one of the leading causes cancer-related mortality worldwide. Here, activity icariin against HCT116 colon cells and possible underlying mechanism were studied. The trypan blue staining assay, wound healing clonogenic CCK-8 Annexin V-FITC/PI double method carried out determine changes cell growth migration. mRNA protein expressions determined by quantitative real-time PCR western blot, respectively. Moreover, small interfering RNA (siRNA) plasmid was used examine role p53 in icariin-induced apoptosis cells. significantly suppressed decreasing migration viability, simultaneously promoting apoptosis. exerted anti-tumor effect a dose-dependent manner up-regulating p53. During treatment icariin, p-p53, p21, Bax levels increased, Bcl-2 level decreased. Short time with induced DNA damage Furthermore, cytotoxicity decreased after knockdown or using caspase inhibitors. involved activities caspase-9 caspase-3. repressed line enhancing expression activating functions possibly through Bcl-2/Bax imbalance -3 regulation. also
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