In Vitro Radioisotopic Labeling of Proteins Associated with Purified Polyoma Virions

摘要: An in vitro procedure using [(14)C]formaldehyde and sodium borohydride was used to label the proteins of purified polyoma virions. This method, which labeled three capsid four internal histones virus, did not alter biological biophysical characteristics virus. In addition seven viral proteins, five additional radioactive peaks were resolved on polyacrylamide gels. Four these could be attributed growing virus presence serum. The fifth peak appears a nonhistone host-contributed protein made before infection.