The effect of bacterial lipopolysaccharides on the biosynthesis and release of proteoglycans from calf articular cartilage cultures.

摘要: Organ cultures of bovine articular cartilage from metacarpophalangeal joints calf maintain steady state metabolism proteoglycans over the course several weeks. Bacterial lipopolysaccharides (LPS) depress biosynthesis in such to approximately 60% control values after 1-2 days treatment. A glycolipid Salmonella minnesota Re 595 mutant, which lacks polysaccharide chains LPS, also depresses proteoglycan synthesis. If LPS is removed medium as late 12 exposure, synthesis returns values. Proteoglycans synthesized during first week treatment are indistinguishable those by terms their hydrodynamic size and relative amounts disaccharides released chondroitin lyase ABC digestion glycosaminoglycan chains. However, 15-18 treatment, significant proportions a smaller synthesized. For prelabeled with [35S]sulfate, rate release 35S-labeled matrix accelerated 2-fold This effect completely reversed upon removal medium. 40 90% lost 18 LPS-treated cultures, respectively. The labeled remaining were newly 7-day when 40% had been lost. Even more than molecules unchanged. stimulates prostaglandin E2 these while indomethacin presence blocks did not alter either or indicating that prostaglandins directly involved regulating this system.