Glycoprotein and ganglioside changes in human trophoblasts after exposure to pulsed Doppler ultrasound
作者： Marija Heffer-Lauc , Višnja Latin , Branko Breyer , Mirna Flögel , Werner E.G. Müller
DOI: 10.1016/0301-5629(94)00131-V
关键词: G(M2) Ganglioside 、 Lectin 、 Glycoprotein 、 Molecular biology 、 Ganglioside 、 Immunology 、 Chemistry 、 Mannose 、 Membrane glycoproteins 、 N-Acetylneuraminic acid 、 Trophoblast
摘要: Changes in glycoprotein and ganglioside composition human trophoblasts (eighth week of gestation) after vitro exposure to pulsed Doppler ultrasound (pulse duration 1.22 microseconds; repetition frequency 11.1 kHz; center 4 MHz; ISPPA = 175.5 W/cm2; ISPTA 0.59 W/cm2) were investigated. Evacuated divided two halves insonated for 10 min on top a 6-cm layer 5% gelatin 50-mL tubes (Falcon) at 37 degrees C. One half each trophoblast was sham served as an internal control. After insonation maintained C 24 h. Glycoproteins detected using alpha-D-mannose specific lectins from Galanthus nivalis Narcissus pseudonarcissus. A decrease the expression mannose containing mgp47 increase mgp54 observed. Ganglioside also significantly altered. Concentrations gangliosides migrating similarly GM2, one GQ1, decreased by more than 75%. At same time, concentrations GM3, other unidentified increased two- fourfold.
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