Long non-coding RNA TDRG1 promotes hypoxia-induced glycolysis by targeting the miR-214-5p/SEMA4C axis in cervical cancer cells.

摘要: Long non-coding RNA (lncRNA) has been demonstrated as vital regulator in human cancer. However, the precise role of lnc-TDRG1 cervical cancer (CC) remains unclear, so this study was aimed to clarify and underlying molecular mechanism CC. The real-time quantitative polymerase chain reaction (RT-qPCR) conducted assess expression levels lnc-TDRG1, miR-214-5p Semaphorin 4C (SEMA4C). Under hypoxia condition, biological behaviors CC cell, including invasion glycolysis were determined by transwell assay Glucose Assay Kit Lactate Kit, respectively. Western blot employed test level SEMA4C hexokinase 2 (HK2) expression. interaction relationship between or analyzed bioinformatics database confirmed dual-luciferase reporter assay, A xenograft experiment nude mice established functional vivo. We found Lnc-TDRG1 highly expressed tissues cells it upregulated response hypoxia. Loss-of-functional suggested that knockdown impede invasion, hypoxia-induced vitro tumor growth vivo, which abolished overexpression SEMA4C. Moreover, we specifically bound negatively correlated with Collectively, regulated sponging mechanistically, could act a sponge regulate SEMA4C, further hypoxia-glycolysis cells.