Genetically Controlled Lysosomal Entrapment of Superparamagnetic Ferritin for Multimodal and Multiscale Imaging and Actuation with Low Tissue Attenuation

摘要: Nanomaterials are of enormous value for biomedical applications because their customizable features. However, the material properties nanomaterials can be altered substantially by interactions with tissue thus making it important to assess them in specific biological context understand and tailor effects. Here, a genetically controlled system is optimized cellular uptake superparamagnetic ferritin subsequent trafficking lysosomes. High local concentrations photoabsorbing magnetoferritin give robust contrast optoacoustic imaging allow selective photoablation cells overexpressing receptors. Genetically biomagnetic nanoparticles also strongly enhances third-harmonic generation due change refractive index caused magnetite–protein interface ferritins entrapped Selective furthermore enables sensitive detection receptor-expressing magnetic resonance imaging, as well efficient cell sorting manipulation. Surprisingly, substantial increase blocking temperature lysosomally observed, which allows ablation defined populations hyperthermia. The subcellular confinement physical empower interrogation processes deep penetration.