Heuristic consequences of a load of oxygen in microtubules.

摘要: Abstract The current cell oxygen paradigm shows some major gaps that have not yet been resolved. Something seems to be lacking for the comprehensive statement of distribution in cell, especially low cytoplasmic level. entrapment microtubules (MTs) resolves latter observation, as well occurrence an extensive foam formation. It leads a novel cells. During steady-state treadmilling, mobile cavity would absorb oxygenated cytoplasm forward, entrap gas nuclei and concentrate them. A fluorescence method is described confirm vitro load MTs during their periodic growths shrinkages. operating mechanism called dynamic instability (GDI) MTs. Several known biosystems could rest on GDI. (1) GTP-cap linked with meniscus encountered tube filled gas. GTP hydrolysis conformational change GTPase domain according bubble pressure, shaking protofilaments particles (soliton-like waves). (2) GDI provides free energy water pump because molecules escape from MT pores when concentrates within MT. Beside ATP motor proteins, additional driving force intracellular transport cargo. streams flowing through slits organize themselves layers between cargo surface, break ionic bridges. makes glide over rail. (3) universal chromosome segregation depolymerization kinetochorial expected generate strong foam. Chromosomes are sucked up pressure difference (or density difference) applied opposite sides kinetochore, which agreement Archimedes’ principle buoyancy. Non-kinetochorial reabsorb Last, mitotic spindle imagined recycler. (4) luminal (called MIPs) organizer, proteins being part borders edges identical bubbles. (5) volatile anesthetics destabilize anesthetic-induced nucleation protofilaments, therefore causing shear stress opening might provide advance this area research.