Purification of oligodendrocyte precursor cells from rat cortices by immunopanning.

摘要: This protocol describes how to purify oligodendrocyte precursor cells (OPCs) from postnatal rodent brains. The method utilizes an immunopanning technique first remove unwanted by negative selection and then OPCs positive subsequent enzymatic release the final panning plate. Included are modifications that allow for purification culturing of mouse instead rat tissue use optic nerves whole isolating brain can be used any specific region brain, provided area dissected away rest tissue. Suggested culture media maintaining proliferating or inducing (OL) differentiation also described.