Epigenomic Analysis of Multi-lineage Differentiation of Human Embryonic Stem Cells

摘要: Epigenetic mechanisms have been proposed as crucial for regulating mammalian development, but their precise function is only partially understood. To investigate the epigenetic control of embryonic we differentiated human stem cells into mesendoderm, neural progenitor cells, trophoblast-like and mesenchymal systematically characterized DNA methylation, chromatin modifications, transcriptome in each lineage. Strikingly, found that promoters are active early developmental stages tend to be CG rich mainly engage H3K27me3 upon silencing non-expressing lineages. By contrast, genes expressed preferentially at later often poor employ methylation repression. Interestingly, regulatory located large genomic domains generally devoid most lineages, termed valleys (DMVs). Our results suggest distinct regulate late ES cell differentiation.