An Acidic PATHOGENESIS-RELATED1 Gene of Oryza grandiglumis is Involved in Disease Resistance Response Against Bacterial Infection
作者： Sang Hyun Shin , Jung-Hun Pak , Mi Jin Kim , Hye Jeong Kim , Ju Sung Oh
DOI: 10.5423/PPJ.NT.11.2013.0112
关键词: Plant disease resistance 、 Open reading frame 、 Microbiology 、 Ectopic expression 、 Suppression subtractive hybridization 、 Complementary DNA 、 Gene 、 Jasmonic acid 、 Arabidopsis thaliana 、 Biology 、 Genetics
摘要: Wild rice, Oryza grandiglumis shows hyper-resistance response to pathogen infection. In order identify genes necessary for defense in plants, we have carried out a subtractive hybridization coupled with cDNA macroarray. An acidic PATHOGENESIS-RELATED1 (PR1) gene of the wild rice is highly identical PR1 different plant species. The OgPR1a has an apparent single open reading frame predicted molecular mass 40,621 Da and isoelectic point 5.14. Both silico analysis transient expression assay onion epidermal cells revealed that protein could be localized intercellular space plants. mRNA was strongly transcribed by exogenous treatment ethylene jasmonic acid as well phosphatase inhibitors. Additionally, ectopic conferred disease resistance on Arabidopsis bacterial fungal infections.
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