Effects of Neighboring DNA homopolymers on the biochemical and physical properties of the Escherichia coli lactose promoter. II. In vitro transcription analyses.
作者： R D Klein , R D Wells
DOI: 10.1016/S0021-9258(18)33609-3
关键词: Recombinant DNA 、 Escherichia coli 、 Wild type 、 DNA sequencing 、 DNA 、 Molecular biology 、 Biochemistry 、 Glycerol 、 lac operon 、 Transcription (biology) 、 Biology
摘要: Transcription studies were conducted on the primary promoter (which initiates at +1) of lac wild type (wt) and UV5 that had insertions poly(dA).poly(dT) or poly(dG).poly(dC) in their -60 region. This series recombinant DNAs was designed to study systematically effect neighboring DNA sequences promoter. Investigations using linear templates showed expression enhanced by insertion, whereas activity not affected this polymer. Neither wt nor influenced presence poly(dG).poly(dC). Quantitation abortive transcripts from supercoiled influence polymers small. Comparative with a secondary near -22), activated 25% glycerol, performed.
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