XRN2 is required for the degradation of target RNAs by RNase H1-dependent antisense oligonucleotides.
作者： Shin-Ichiro Hori , Tsuyoshi Yamamoto , Satoshi Obika
DOI: 10.1016/J.BBRC.2015.06.171
关键词: RNase P 、 Biology 、 RNA 、 Mature messenger RNA 、 Oligonucleotide 、 Molecular biology 、 Messenger RNA 、 Gene 、 Exoribonuclease 、 Cleavage (embryo)
摘要: Antisense oligonucleotides (ASOs) can suppress the expression of a target gene by cleaving pre-mRNA and/or mature mRNA via RNase H1. Following initial endonucleolytic cleavage H1, RNAs are degraded mechanism that is poorly understood. To better understand this degradation pathway, we depleted two major 5' to 3' exoribonucleases (XRNs), named XRN1 and XRN2, analyzed levels fragments in vitro. We found generated ASO were almost completely from their ends nuclear XRN2 after H1-mediated cleavage, whereas partially XRN2. In contrast ASO, small interference RNA (siRNA) could reduce level only mRNA, fragment was cytoplasmic XRN1. Our findings indicate targeted H1-dependent rapidly nucleus, contrary pathway mediated siRNA.
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