Micro-mechanical sensing platform for the characterization of the elastic properties of the ovum via uniaxial measurement

摘要: Abstract Stiffness is an important parameter in determining the physical properties of living tissue. Recently, considerable biomedical attention has centered on mechanical tissues at single cell level. In present paper, Young’s modulus zona pellucida bovine ovum was calculated using Micro Tactile Sensor (MTS) fabricated piezoelectric (PZT) material. The sensor consists a needle-shaped 20-μm transduction point made micro-electrode puller and mounted micro-manipulator platform. Measurements were under microscopic control, suction pipette to support same horizontal axis as MTS. found be 25.3±7.94 kPa ( n =28). This value indirectly determined based calibration curves relating change resonance frequency (Δ f 0 ) with tip displacement for gelatin concentrations 4%, 6%, 8%. regression equation between rate (versus displacement), Δ / x (Hz/μm)=0.2992×Young’s (kPa)−1.0363. It concluded that reason stiffness measured study approximately six times larger than previously reported, may due absence large deformation existing methodologies.