Different activation domains stimulate transcription from remote ('enhancer') and proximal ('promoter') positions.

摘要: We reported previously that the lymphocyte-derived octamer transcription factor 2A (Oct-2A or OTF-2A) activated both natural immunoglobulin promoters and synthetic which contain 'octamer' site, but was unable by itself to stimulate from a remote enhancer position. Here we examine larger set of factors with respect their proximal versus activation. Since may more than one activation domain, have chosen study potential individual domains in context fusion proteins DNA binding domain GALA. identified at least two distinct functional classes transcriptional domains. 'Proximal' domains, exemplified glutamine-rich Oct-1, Oct-2A Sp1, only position close TATA box, usually response enhancer. 'General' derived VP16, GAL4, p65 (NF-chi B), TFE3, ITF-1 ITF-2, can activate as well positions. These many acidic amino acids and/or other features such clusters serine threonine. The proline-rich AP-2 CTF/NF1 represent third class considerable promoter activity low significant activity. Furthermore, types seem modular structure, since duplicated subdomains substitute for entire domain.