Self-enhanced electrochemiluminescence immunosensor based on nanowires obtained by a green approach.

摘要: Abstract Co-reactant electrochemiluminescence (ECL) is a simple and effective method for sensitive detection with amplified ECL signals. However, the intermolecular interaction between luminescent reagents their corresponding co-reactants, which widely applied, has disadvantages in poor stability, low efficiency of electron transfer relatively high loss energy. In this work, an intramolecular self-enhanced proposed to settle problem. Firstly, palladium nanowires (PdNWs) are synthesized green procedure Lentinan (LNT), one β-glucans triple helical conformation (t-LNT) aqueous solution single chains (s-LNT) at temperature higher than 130 °C, used as stabilizer reducing agent. The abtined PdNWs applied immobilize polyamidoamine (PAMAM) dendrimer further reacts tris (4, 4′-dicarboxylicacid-2, 2′-bipyridyl) ruthenium (II) dichloride form new electrochemiluminescent derivative (PdNWs–PAMAM–Ru). way, Ru luminophore its co-reactive groups (amine PAMAM) exist same complex, by electronic transmission distance shortened luminous properties including stability enhanced. Moreover, due specific surface areas good electro-catalytic ability PdNWs, obtained PdNWs–PAMAM–Ru can be also antibody (Ab 2 ). Then, sandwiched immunosensor fabricated carcinoembryonic antigen (CEA) wide linear ranged from 0.001 ng mL −1 80 ng mL limit 0.3 pg mL .