Activated complement components and complement activator molecules on the surface of cell-derived microparticles in patients with rheumatoid arthritis and healthy individuals

摘要: Objectives: In vitro, microparticles can activate complement via the classical pathway. If demonstrable ex vivo, this mechanism may contribute to pathogenesis of rheumatoid arthritis (RA). We therefore investigated presence activated components and activator molecules on surface cell-derived RA patients healthy individuals. Methods: Microparticles from synovial fluid (n = 8) plasma (n = 9) 10 sex- age-matched individuals (n = 10) were analysed by flow cytometry for bound (C1q, C4, C3) (C-reactive protein (CRP), serum amyloid P component (SAP), immunoglobulin (Ig) M, IgG). Results: with C1q, and/or C3 abundant in fluid, while control much lower levels present. C1q correlated those (r = 0.961, p = 0.0001), C4 (RA: r = 0.908, p = 0.0007; control: r = 0.632, p = 0.0498), indicating pathway activation. IgM IgG ( r  = 0.728, p = 0.0408; r = 0.952, p = 0.0003, respectively), plasma, CRP r = 0.903, p = 0.0021; r = 0.683, p = 0.0296), implicating activation low level plasma. Conclusions: This study demonstrates supports their role grade increased fluid.