Supplementation of Jurkat T Cells with Green Tea Extract Decreases Oxidative Damage Due to Iron Treatment

摘要: Regular tea consumption has been associated with a reduced risk of cancer. As demonstrated in vitro, green contains catechins antioxidant properties. We evaluated the effect supplementation Jurkat T-cell line extract on oxidative damage. Cells grown medium or without (10 mg/L) were treated Fe(2+) (100 micromol/L) as an stimulus for 2 h. Cell membrane lipid peroxidation was by fatty acids pattern analysis and malondialdehyde production alpha-linolenic acid-loaded cells. Furthermore, DNA damage (single strand breaks) detected cells Comet assay quantified relative tail moment (RTM). Supplementation significantly decreased (1.6 +/- 0.3 vs. 0.6 0.1 nmol/mg protein, P < 0.05) (0.32 0.07 0.12 0.04 RTM, after treatment. In control cells, there no distribution (n-3) due to enrichment acid increased total acids. However, treatment did not modify polyunsaturated It is likely that observed protective effects can be attributed epigallocatechin gallate, which present mainly (670 g/kg) extract; however, we cannot exclude contributions other catechins. These data support against