Fibrin sheath enhances central venous catheter infection.

摘要: OBJECTIVE: To determine whether fibrin-coated central venous catheters have a higher infection rate, and spawn more septic emboli, than uncoated after exposure to bacteremia. DESIGN: Animal study comparing catheter blood cultures of exposed SETTING: laboratory. SUBJECTS: Adult male Sprague-Dawley rats. INTERVENTIONS: A total 210 rats had placed with the proximal end buried subcutaneously. Rats were divided into three groups: tail vein bacterial injection on day 0 (no fibrin group) or 10 (fibrin group), no injection/saline (control, n = 40). Bacterial injections 1 x 108 colony forming units either Staphylococcus epidermidis (n 100) Enterobacter cloacae 60). Animals killed 3 days injection. Blood obtained via cardiac puncture, removed chest. Half was rolled onto agar other half in trypticase soy broth. Plates broth incubated at 37 degrees C for 48 hrs. The presence >15 colonies roll plates, growth broth, accepted as positive sign infection. Microscopy performed 20-10 catheters. Thirty animals without underwent culture MEASUREMENTS AND MAIN RESULTS: Catheter S. occurred 32% plates 80% from group vs. 4% 20% (p <.01 each). E. 50% 0% 12% Positive 47 68 8 <.01). showed sheath 20 Without catheters, 30 negative. CONCLUSION: significantly enhanced catheter-related persistent