Incorporation of 32P-labelled intermediates into the phospholipids of cell-free preparations of rat brain.

摘要: In previous papers (McMurray, Strickland, Berry & Rossiter, 1957b; McMurray, 1957a) two systems capable of supporting the incorporation inorganic 32p into phospholipids rat-brain dispersions were described: (a) an anaerobic system (best demonstrated in hypotonic dispersions), which supported labelling lipid P under optimum conditions for glycolysis (McMurray etal. 1957 b), and (b) aerobic shown mitochondria isolated from isotonic oxidative phosphorylation et al. a). The effects metabolia inhibitors such as 2:4-dinitrophenol (DNP) fluoride on specific radioactivity concentration adenosine triphosphate (ATP) each these suggested thlat phospholipid was dependent upon both prior ofATP maintenance adequate concentrations this substance order to elucidate some intermediate reactions concerned with 82p, a study made of82P-labelledadenosinetriphosphate (AT22P), phosphorylcholine (32PCh) a-glycerophosphate (a-G82P) phosphorylating systems. distribution hydrolysis products individual phospholipids, separated by chromatographic method Dawson (1954b), that 82PCh oc-G82P incorporated lecithin phosphatidic acid respectively. Inorganic 32P appeared be chiefly diphosphoinositide. A preliminary account work has 1956).