Transcriptome Profiling of Panc-1 Spheroid Cells with Pancreatic Cancer Stem Cells Properties Cultured by a Novel 3D Semi-Solid System.

摘要: Background/Aims: Pancreatic cancer remains one of the deadliest human malignancies, lethality which may be attributed to presence pancreatic stem cells (PCSCs), a small subpopulation existing within tumor with high carcinogenesis. Therefore, it is crucial establish an efficient enrichment and culture system PCSCs identify key genes involved in regulation PCSCs. The three-dimensional (3D) liquid suspension mammosphere has been established for vitro as cell spheres are likely originate from individual clone, but challenged because spheroids could result aggregation. Methods: We optimized by adding methylcellulose create 3D semi-solid prevented non-specific Then we identified CSC properties Panc-1 spheroid cultured this detecting associated stemness evaluation tumorigenicity vivo through invasion, migration xenograft experiments methods. Subsequently, performed high-throughput sequencing (HTS) cells. Results: confirmed malignancy enriched our novel both vivo. Hundreds mRNA, microRNA (miRNA) dozens long non-coding RNA (LncRNA) were differentially regulated PCSCs-like compared their parental HTS. Conclusions: Our results demonstrate properties. expressed targets HTS can serve new potential biomarkers diagnosis targeted therapy.