Three-dimensional imaging of whole mouse models: comparing nondestructive X-ray phase-contrast micro-CT with cryotome-based planar epi-illumination imaging

摘要: In this study, we compare two evolving techniques for obtaining high-resolution 3D anatomical data of a mouse specimen. On the one hand, investigate cryotome-based planar epi-illumination imaging (cryo-imaging). other examine X-ray phase-contrast micro-computed tomography (micro-CT) using synchrotron radiation. Cryo-imaging is technique in which an electron multiplying charge coupled camera takes images cryo-frozen specimen during sectioning process. Subsequent image alignment and virtual stacking result volumetric data. based on minute refraction X-rays inside features higher soft-tissue contrast than conventional, attenuation-based micro-CT. To explore potential both studying whole disease models, was imaged techniques. Obtained are compared visually quantitatively, specifically with regard to visibility fine details. Internal structure visible great detail study shows particular that strongly enhanced phase images. This identifies micro-CT as powerful tool small animal models.