Characterization of the promoter region of the cystic fibrosis transmembrane conductance regulator gene.
作者： J.L. Chou , R. Rozmahel , L.C. Tsui
DOI: 10.1016/S0021-9258(18)54252-6
关键词: Response element 、 Negative regulatory element 、 Regulatory sequence 、 Upstream activating sequence 、 Promoter 、 Cystic fibrosis transmembrane conductance regulator 、 Biology 、 Enhancer 、 Reporter gene 、 Molecular biology
摘要: To identify the transcription regulatory elements of cystic fibrosis transmembrane conductance regulator (CFTR) gene, DNA fragments located in 5‘-upstream region were fused with bacterial chloramphenicol acetyltransferase (CAT) reporter gene and transfected into various cell lines to test for promoter activity. The results these studies suggested that there at least two positive one negative cisacting involved CFTR initiation. One them was a proximal, element delimited by 5‘ deletion constructs -226 base parts upstream start site. This minimal sequence (-226 +98) alone seemed be sufficient direct cell-specific CAT expression. sequences immediately -227, on other hand, appeared contain element; inclusion this proximal (e.g. construct containing -345 rendered inactive. could also suppress activity heterologous promoter. In addition, transfection study existence another outside examined, as inability -658 function assay overcome presence viral enhancer element.
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