siRNA targeting TCTP suppresses osteosarcoma cell growth and induces apoptosisin vitroandin vivo

摘要: Osteosarcoma (OS) remains the most frequent primary malignant bone tumor in adolescents. However, molecular cause of disease is poorly elucidated. In present study, we primarily found that translationally controlled protein (TCTP) was overexpressed human OS tissues and cell lines. To investigate function TCTP growth, an RNA interference lentivirus system employed to deplete expression Saos-2 U2OS Specific knockdown significantly impaired proliferation colony-formation capacity both Moreover, depletion caused a significant accumulation cells S phase eventually induced apoptosis. Expression levels G2/M regulators cyclin B1 Cdc25A were decreased, apoptotic markers Bad caspase-3 increased lines after TCTP. Furthermore, potently inhibited growth xenografts nude mice. Our results indicate inhibition exerts potential antitumor activity may be novel therapeutic approach OS.