Development of a fast ELISA for quantifying polio D-antigen in in-process samples.
作者： R. ten Have , Y.E. Thomassen , M.R.J. Hamzink , W.A.M. Bakker , O.E.M. Nijst
DOI: 10.1016/J.BIOLOGICALS.2011.11.004
关键词: Reagent 、 Molecular biology 、 Antigen 、 Chemistry 、 Chromatography
摘要: A fast ELISA was developed and qualified for analysis of polio D-antigen. The original 20 h-protocol optimized by minimizing the total incubation time to 1 h, replacing signal reagent 3,3',5,5'-tetramethylbenzidine a chemiluminogenic with theoretical low intrinsic background high dynamic range.
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