Reduction of telomeric signals in murine melanoma and human breast cancer cell lines treated with 3'-azido-2'-3'-dideoxythymidine

摘要: The purpose of this study was to determine, using fluorescence in situ hybridization (FISH), whether treatment cancer cells with 3'-azido-2'-3'-dideoxythymidine (AZT) has any effect on telomere length as determined by the telomeric signal intensity. To do so, we treated a metastatic murine melanoma cell line (K-1735 clone X-21) and human breast (MCF-7) three concentrations AZT for 72 h at 37uC. FISH preparations processed an all-human DNA probe showed significantly reduced, concentration-dependent intensity interphase metaphase spreads AZT-treated compared untreated controls, which no reduction. We conclude from these preliminary results that potential targeting ends chromosomes promoting death could well be tested along other chemotherapy drugs given patients purpose.