Absence of alternative splicing in bcr-abl mRNA in chronic myeloid leukemia cell lines.
作者： A Hermans , L Selleri , J Gow , GC Grosveld
DOI: 10.1182/BLOOD.V72.6.2066.2066
关键词: Alternative splicing 、 Cancer research 、 splice 、 Cell culture 、 Myeloid leukemia 、 Biology 、 Transition (genetics) 、 Oncogene 、 Messenger RNA 、 Chromosomal translocation 、 Virology
摘要: The major consequence of the Philadelphia (Ph) translocation in chronic myeloid leukemia (CML) is formation a bcr-abl hybrid oncogene encoding tumor cell-specific protein P210bcr-abl. In contrast to this, Ph chromosome-positive acute lymphoblastic (Ph + ALL), P190bcr-abl can be observed. This has been implicated rather than leukemogenesis. Therefore, it hypothesized that transition from blast phase CML accompanied by an alternative splice mRNA, which results switch production P210bcr-abl into P190bcr-abl. Initial S1 nuclease protection mapping supported this theory. However, result appears based on artifact analysis. By using polymerase chain reaction we provide evidence for absence splicing mRNA two crisis cell lines.
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