Cadmium inhibits molting of the freshwater crab Sinopotamon henanense by reducing the hemolymph ecdysteroid content and the activities of chitinase and N-acetyl-β-glucosaminidase in the epidermis

摘要: Molting is an essential process during the growth of crustaceans, which coordinated by ecdysteroids secreted Y-organ, molting inhibiting hormone X-organ sinus-gland complex, as well chitinase and N-acetyl-β-glucosaminidase synthesized epidermis. Cadmium one toxic metals in aquatic environment. However, endocrine effects cadmium on freshwater crabs underlying mechanisms are unknown. To investigate these, (Sinopotamon henanense) were acutely exposed to 0, 7.25, 14.5 29 mg/l Cd for 3, 4, 5 days or some experiments 4 after eyestalk-ablation. The concentration hemolymph ecdysone activities enzymes NAG measured. Histological changes epidermal tissues documented. Our results showed that eyestalk ablation increased ecdysteroid content NAG, inhibited a concentration-dependent manner; histological examinations demonstrated produced storage particles tissues, was also reduced manner. data suggest disrupts function through secretion Y-organ altering with regulation activity This work provides new insights into inhibition effect crabs.