Novel ribosomal mutations affecting translational accuracy, antibiotic resistance and virulence of Salmonella typhimurium

摘要: Many mutations in rpsL cause resistance to, or dependence on, streptomycin and are restrictive (hyperaccurate) translation. Dependence on hyperaccuracy can each be reversed phenotypically by either rpsD rpsE. Such compensatory have been shown to a ram phenotype (ribosomal ambiguity), increasing the level of translational errors. We recently that alleles also associated with loss virulence Salmonella typhimurium. To test whether mutants could reverse this virulence, we isolated set found restore strains carrying close wild type. Unexpectedly, three out seven mutant tested phenotypes typical rpsL, being resistant These not previously ribosomal protein S4. Furthermore, all (four restrictive) rpsL. This is first demonstration such compensations do require compensating allele has ambiguity (ram) phenotype.