Investigation of trypsin-CdSe quantum dot interactions via spectroscopic methods and effects on enzymatic activity
作者： Gurvir Kaur , S.K. Tripathi
DOI: 10.1016/J.SAA.2014.05.064
关键词: Cadmium selenide 、 Binding constant 、 Quenching (fluorescence) 、 Photochemistry 、 Acceptor 、 Quantum dot 、 Trypsin 、 Förster resonance energy transfer 、 Chemistry 、 Analytical chemistry 、 Fluorescence
摘要: The paper presents the interactions between trypsin and water soluble cadmium selenide (CdSe) quantum dots investigated by spectrophotometric methods. CdSe have strong ability to quench intrinsic fluorescence of a static quenching mechanism. has been studied at three different temperatures where results revealed that electrostatic exist are responsible stabilize complex. Scatchard plot from 1 binding site for trypsin, same confirmed making isothermal titrations against trypsin. distance donor acceptor trypsin-CdSe dot complexes is calculated be 2.8 nm energy transfer mechanisms. also enhanced linear concentration ranging 1-80 μl. All observations evidence formation conjugates, retains enzymatic activity which in turn temperature pH dependent.
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sci-hub.se 参考文章(53)
Toshiaki Hiratsuka, Conformational changes in the 23-kilodalton NH2-terminal peptide segment of myosin ATPase associated with ATP hydrolysis. Journal of Biological Chemistry. ,vol. 265, pp. 18786- 18790 ,(1990) , 10.1016/S0021-9258(17)30581-1
Neil D. Rawlings, Alan J. Barrett, Families of serine peptidases. Methods in Enzymology. ,vol. 244, pp. 19- 61 ,(1994) , 10.1016/0076-6879(94)44004-2
Mingyong Han, Xiaohu Gao, Jack Z. Su, Shuming Nie, Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules Nature Biotechnology. ,vol. 19, pp. 631- 635 ,(2001) , 10.1038/90228
Glenn A. Crosby, James N. Demas, Measurement of photoluminescence quantum yields. Review The Journal of Physical Chemistry. ,vol. 75, pp. 991- 1024 ,(1971) , 10.1021/J100678A001
M. R. Eftink, C. A. Ghiron, Exposure of tryptophanyl residues in proteins. Quantitative determination by fluorescence quenching studies. Biochemistry. ,vol. 15, pp. 672- 680 ,(1976) , 10.1021/BI00648A035
Wengui Chang, Yuhua Shen, Anjian Xie, Hui Zhang, Juan Wang, Wensheng Lu, Controlled synthesis of CdSe and CdSe/CdS core/shell nanoparticles using Gemini surfactant Py-16-10-16 and their bioconjugates with BSA. Journal of Colloid and Interface Science. ,vol. 335, pp. 257- 263 ,(2009) , 10.1016/J.JCIS.2009.03.035
Reinskje Talhout, Alessandra Villa, Alan E. Mark, Jan B. F. N. Engberts, Understanding binding affinity: a combined isothermal titration calorimetry/molecular dynamics study of the binding of a series of hydrophobically modified benzamidinium chloride inhibitors to trypsin. Journal of the American Chemical Society. ,vol. 125, pp. 10570- 10579 ,(2003) , 10.1021/JA034676G
Al. L. Efros, M. Rosen, M. Kuno, M. Nirmal, D. J. Norris, M. Bawendi, Band-edge exciton in quantum dots of semiconductors with a degenerate valence band: Dark and bright exciton states Physical Review B. ,vol. 54, pp. 4843- 4856 ,(1996) , 10.1103/PHYSREVB.54.4843
Debashis Banerjee, Parimal Kumar Das, Haraprasad Singha, Sanjib Bagchi, Fluorimetric study of interaction of merbromin with trypsin. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. ,vol. 70, pp. 1109- 1113 ,(2008) , 10.1016/J.SAA.2007.10.019
Ana Catarina C Esteves, Tito Trindade, Synthetic studies on II/VI semiconductor quantum dots Current Opinion in Solid State & Materials Science. ,vol. 6, pp. 347- 353 ,(2002) , 10.1016/S1359-0286(02)00079-7