The sustained release of basic fibroblast growth factor accelerates angiogenesis and the survival of the inactivated dermis by high hydrostatic pressure

摘要: We developed a novel skin regeneration therapy combining nevus tissue inactivated by high hydrostatic pressure (HHP) in the reconstruction of dermis with cultured epidermal autograft (CEA). The issue this treatment is unstable survival CEA on dermis. In study, we applied collagen/gelatin sponge (CGS), which can sustain release basic fibroblast growth factor (bFGF), to order accelerate angiogenesis. Murine grafts from C57BL6J/Jcl mice (8 mm diameter) were prepared, HHP and cryopreserved. One month later, transplanted subcutaneously onto back other covered CGS impregnated saline or bFGF. Grafts taken after one, two eight weeks, at point was evaluated through histology angiogenesis-related gene expressions determined real-time polymerase chain reaction. Histological sections showed that dermal cellular density newly formed capillaries bFGF group significantly higher than control group. relative expression FGF-2, PDGF-A VEGF-A genes Week 1. This study suggested angiogenesis into accelerated, might improve combination sustained CGSs.