Folic acid deficiency impairs the gill health status associated with the NF-κB, MLCK and Nrf2 signaling pathways in the gills of young grass carp (Ctenopharyngodon idella).
作者： Lei Shi , Lin Feng , Wei-Dan Jiang , Yang Liu , Jun Jiang
DOI: 10.1016/J.FSI.2015.09.023
关键词: Fish gill 、 Enzyme 、 Glutathione reductase 、 Biochemistry 、 Endocrinology 、 Glutathione 、 Biology 、 Internal medicine 、 Acid phosphatase 、 Grass carp 、 Antioxidant 、 Glutathione peroxidase
摘要: The aim of this study was to investigate the effect dietary folic acid on fish growth, immune and barrier functions gills, potential mechanisms these effects. Young grass carp (Ctenophatyngodon idella) were fed diets containing graded levels at 0.10 (basal diet), 0.47, 1.03, 1.48, 1.88 3.12 mg kg(-1) diet for 8 weeks. results showed that phosphatase lysozyme activities complement component 3 content in gills decreased with deficiency (P < 0.05). Folic up-regulated liver-expressed antimicrobial peptide 1, interleukin 1 beta, 8, tumor necrosis factor alpha, nuclear kappa B p65, I kinase alpha (IKK-alpha), IKK-beta IKK-gamma gene expression. down-regulated 10, transforming growth target rapamycin expression These limited gill status. Furthermore, claudin-b, claudin-c, claudin-3, occludin zonula occludens expression, whereas claudin-12, claudin-15, myosin light chain p38 mitogen activated protein suggested disrupted tight junction-mediated function. Additionally, increased reactive oxygen species, carbonyl malondialdehyde (MDA); Mn superoxide dismutase activity expression; Kelch-like-ECH-associated 1a (Keap1a) Keap1b Conversely, Cu/Zn dismutase, catalase, glutathione peroxidase, s-transferases reductase as well NF-E2-related 2 All indicated impaired health status via regulating cytokines, junction proteins, antioxidant enzymes, NF-kappa MLCK Nrf2. Based percent weight gain, LZ MDA requirements young 1.60, 2.07 2.08 kg(-1), respectively. (C) 2015 Published by Elsevier Ltd.
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