Enhancing the chondrogenic potential of chondrogenic progenitor cells by deleting RAB5C.
作者： Valerio Izzi , Vicki Rosen , Elvira Henze , Nicolai Miosge , Christof Lenz
DOI: 10.1016/J.ISCI.2021.102464
关键词: SOX9 、 Cartilage 、 Matrix (biology) 、 Cell biology 、 Chemistry 、 RUNX2 、 Gene knockdown 、 Progenitor cell 、 Chondrogenesis 、 Osteoarthritis
摘要: Summary Osteoarthritis (OA) is the most prevalent chronic joint disease that affects a large proportion of elderly population. Chondrogenic progenitor cells (CPCs) reside in late-stage OA cartilage tissue, producing fibrocartilaginous extra-cellular matrix; these can be manipulated vitro to deposit proteins healthy articular cartilage. CPCs are under control SOX9 and RUNX2. In our earlier studies, we showed knockdown RUNX2 enhanced chondrogenic potential CPCs. Here demonstrate carrying knockout RAB5C, protein involved endosomal trafficking, exhibited elevated expression multiple markers, including SOX trio, increased COL2 deposition, whereas no changes COL1 deposition were observed. We report RAB5C as an attractive target for future therapeutic approaches designed increase content diseased joint.
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