Deploying clinical grade magnetic nanoparticles with magnetic fields to magnetolabel neural stem cells in adherent versus suspension cultures

摘要: Neural stem cells (NSCs) have a high therapeutic potential for patients with neurological disease/injury given their neuroregenerative and immunomodulatory capabilities. In recent years, magnetic nanoparticles (MNPs) been used as contrast agents in translational studies, to track transplanted NSCs using non-invasive resonance imaging (MRI). However, NSC uptake of MNPs is inherently low the absence chemical/biological enhancing strategies such cell targeting peptides transfection – approaches which may be cytotoxic alter cellular physiology. By contrast, physical delivery relying on assistive methods can safely enhance MNP into multiple neural types. The utility this approach has demonstrated gene grade particles but application ‘magnetolabelling’ clinical never evaluated. Here, we show that applied oscillating fields (Lumirem/Ferumoxsil) propagated neurospheres (suspension cultures, preferred format transplantation) offer limited benefit monolayer (adherent) cultures. This method therefore facilitate labelling therapies.