Engineering bacterium for extracellular secretory expression of inonotus obliquus dipeptidase

摘要: The invention relates to an engineering bacterium for extracellular secretory expression of inonotus obliquus dipeptidase. By operably connecting a cloned dipeptidase gene IDP in transmembrane region with vector obtain recombinant capable expressing the protein, and introducing into proper host cell. also provides method preparing IDP, which comprises following steps: culturing IDP-X33, carrying out methanol induced region. culture conditions are as follows: temperature is 28 DEG C, initial pH value 7, time 96 hours at 200 rpm, induction condition that inducer supplemented once every 24 until final concentration 1.0%. According by pichia pastoris provided invention, activity secretase reaches 24U/mL, cell density (OD 600) 24.5, secretion efficiency enzyme 1U/mL.